上海体育学院学报

2019, (06) 66-72

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游泳对SHR大鼠血压、心功能及心肌PRMT-1/ADMA-DDAH-2/Cit-eNOS/NO通路的影响
Effect of Swimming on Blood Pressure and Cardiac Function and PRMT-1/ADMA-DDAH-2/Cit-eNOS/NO Pathway in SHR Rats

潘孝贵;金其贯;李蕾
PAN Xiaogui; JIN Qiguan; LI Lei

摘要(Abstract):

目的 探讨游泳运动对自发性高血压(SHR)大鼠心功能及心肌PRMT-1/ADMA-DDAH-2/Cit-eNOS/NO通路的影响。方法 50只SHR大鼠随机分为0周对照组(C-0)、4周对照组(C-4)、8周对照组(C-8)、4周运动组(E-4)、8周运动组(E-8),运动组进行连续4周、8周的游泳运动后,测定大鼠血压、心功能、心肌组织ADMA、Cit、L-Arg、NO含量,以及eNOS活性、PRMT-1和DDAH-2 mRNA。结果 与C-0组比较,C-4、C-8组SBP、DBP、LVM、LVEDV、LVESV、SV显著升高,C-8组心肌ADMA、PRMT-1 mRNA显著升高,Cit和L-Arg/ADMA比值、DDAH-2 mRNA显著降低,但E-4、E-8组SBP、DBP、LVM、LVESV,以及心肌PRMT-1 mRNA显著降低,EF、FS、LVEDV、SV,以及DDAH-2 mRNA显著升高,E-4组心肌Cit显著降低,NO显著升高(P<0.01),E-8组ADMA显著降低,L-Arg、NO含量,以及L-Arg/ADMA比值和eNOS活性显著升高。与同周龄对照组比较,E-4、E-8组SBP、DBP、LVM、LVESV显著低于C-4、C-8组,EF、FS、SV显著高于C-4、C-8组,E-4组Cit显著降低,NO、DDAH-2 mRNA显著升高,E-8组Cit、ADMA、PRMT-1 mRNA显著降低,L-Arg、NO含量,以及L-Arg/ADMA比值和eNOS活性、DDAH-2 mRNA显著升高。E-8组与E-4比较,FS、LVM、LVEDV、LVESV、Cit、NO含量,以及L-Arg/ADMA比值和eNOS活性显著升高,ADMA、PRMT-1 mRNA显著降低。结论 运动通过下调SHR心肌PRMT-1和/或上调DDAH-2基因表达,减少ADMA堆积,提高L-Arg/ADMA比值,增强eNOS活性,促进NO合成,从而改善心功能,促使血压降低。
Objective: To investigate the effects of swimming on cardiac function and PRMT-1/ADMA-DDAH-2/Cit-eNOS/NO pathway in spontaneously hypertensive rats (SHR).Methods: Fifty SHR rats were randomly divided into 0-week control group (C-0),4-week control group (C-4),8-week control group (C-8),4-week exercise group (E-4),8-week exercise group (E-8).After swimming for 4 or 8 weeks,the blood pressure,cardiac function,ADMA,Cit,L-Arg,NO contents,eNOS activity and PRMT-1,DDAH-2 mRNA expression in myocardium were measured.Results: (1) Compared with C-0 group,SBP,DBP,LVM,LVEDV,LVESV,and SV increased significantly in C-4 and C-8 groups,and in C-8 group,ADMA,PRMT-1 mRNA increased remarkably,while Cit,L-Arg/ADMA,DDAH-2 mRNA decreased significantly.However,in E-4 and E-8 groups,SBP,DBP,LVM,LVESV and PRMT-1 mRNA decreased significantly,while EF,FS,LVEDV,SV,and DDAH-2 mRNA increased significantly,and in E-4 group,Cit decreased significantly,while NO increased significantly,and in E-8 group,ADMA decreased significantly,while L-Arg,NO,L-Arg/ADMA,eNOS activity increased significantly.(2) Compared with the age-matched C-4 and C-8 groups,SBP,DBP,LVM,LVESV were significantly lower, while EF,FS,SV significantly higher,in E-4 and E-8 groups.In E-4 group,Cit was significantly lower,while NO,DDAH-2 mRNA were significantly higher,and in E-8 group,Cit,ADMA,PRMT-1 mRNA were significantly lower,while L-Arg,NO,L-Arg/ADMA,eNOS,DDAH-2 mRNA were significantly higher.(3) Compared with E-4 group,FS,LVM,LVEDV,LVESV,Cit,NO,L-Arg/ADMA,and eNOS activity increased significantly,while ADMA,PRMT-1 mRNA decreased significantly in E-8 group.Conclusion: Exercise can reduce ADMA accumulation,increase L-Arg/ADMA ratio and enhance eNOS activity and NO synthesis by down-regulating PRMT-1 and/or up-regulating DDAH-2 gene expression in myocardium,thus enhancing cardiac function and contributing to lowering blood pressure SHR.

关键词(KeyWords): SHR大鼠;游泳训练;血压;心功能;内皮一氧化氮合成酶
SHR rats;swimming training;blood pressure;cardiac function;endothelial nitric oxide synthase(eNOS)

Abstract:

Keywords:

基金项目(Foundation): 西华师范大学博士科研启动基金资助项目(18Q014)

作者(Author): 潘孝贵;金其贯;李蕾
PAN Xiaogui; JIN Qiguan; LI Lei

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